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KMID : 1161420100130061431
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Journal of Medicinal Food
2010 Volume.13 No. 6 p.1431 ~ p.1442
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Preparation of Carotenoids and Chlorophylls from Gynostemma pentaphyllum (Thunb.) Makino and Their Antiproliferation Effect on Hepatoma Cell
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Tsai Yu-Chian
Wu Wen-Bin
Chen Bing-Huei
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Abstract
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A preparative column chromatographic method for isolation of carotenoids and chlorophylls from Gynostemma pentaphyllum, a traditional Chinese herb, was developed to evaluate their antiproliferative effects on the hepatoma cell Hep3B. An open column containing 70?g of magnesium oxide?diatomaceous earth (1:2.5, wt/wt) was used to elute carotenoid with 2% ethanol in ethyl acetate and chlorophyll with 50% ethanol in acetone. After high-performance liquid chromatography?mass spectrometry analysis, the carotenoid fraction was composed of all-trans- and cis-isomers of lutein, α-carotene, and β-carotene as well as epoxy-containing carotenoids, while the chlorophyll fraction consisted of chlorophylls a and b and their derivatives. Both carotenoid and chlorophyll fractions as well as lutein and chlorophyll a standards at 50?100?μg/mL were effective against Hep3B cells with a dose-dependent response with the following order: carotenoid fraction?>?chlorophyll fraction?>?lutein?>?chlorophyll a. For all treatments, the cell cycle was arrested in the G0/G1 phase, with Hep3B cells undergoing necrosis or apoptosis.
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KEYWORD
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carotenoid, cell cycle analysis, chlorophyll, column chromatography, Gynostemma pentaphyllum
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